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Isolation of Monocyte-Macrophage Lineage Cells from Rat Bones by Secondary Adherence Method
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Isolation of Monocyte-Macrophage Lineage Cells from Rat Bones by Secondary Adherence Method

Isolation of Monocyte-Macrophage Lineage Cells from Rat Bones by Secondary Adherence Method

DOI:
10.3791/64053-v

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04:19 min

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July 13, 2022

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, , , ,
1School of Medical Technology and Information Engineering,Zhejiang Chinese Medical University, 2School of Basic Medical Sciences,Fudan University

Chapters

  • 00:04Introduction
  • 00:59Cell Extraction
  • 02:37Results: Isolation of Mononuclear Macrophages from the Bone Marrow
  • 03:36Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol for the isolation of BMMs from SD rats, called the secondary adherence method.

Tags

Keywords: Monocyte-macrophageBone MarrowOsteoclastSecondary AdherenceCell IsolationCell CultureTRAP StainFlow CytometryCD11b/c

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