Ovarian Surface Epithelium
Ovarian Surface Epithelium - Related Videos
07:37
Human Ovarian Surface Epithelium Organoids as a Platform to Study Tissue Regeneration
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Cited by 4 •
2024
This protocol describes establishing three-dimensional (3D) tissue organoids from primary human ovarian surface epithelium (hOSE) cells. The protocol includes isolation of hOSE from freshly collected ovaries, cellular expansion of the hOSE, cryopreservation-thawing procedures, and organoid derivation. Immunofluorescence, quantitative analysis, and showcasing utility as a screening platform are included.
10:35
An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses
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Cited by 18 •
2010
To study in vivo tumor growth and tumor microenvironment, we used a syngeneic and orthotopic mouse model of ovarian cancer in immunocompetent animals. We transduced a mouse tumor cell line (MOV1) with Katushka fluorescent protein (MOV1KAT) and here we show its orthotopic implantation in ovary and in vivo imaging.
09:25
Transplantation Into the Mouse Ovarian Fat Pad
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Cited by 7 •
2016
We describe an ovarian fad pad transplantation assay suitable for studies of normal and transformed epithelia of the female reproductive tract. The mouse fat pad allows transplantation of large tissue fragments, is easily accessible for surgery and imaging, and provides the most favorable native environment for tissues of Müllerian origin.
08:09
Tubal Cytology of the Fallopian Tube as a Promising Tool for Ovarian Cancer Early Detection
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Cited by 11 •
2017
We explored a tubal cytologic method by sampling the fallopian tube directly post-surgical excision as a tool of ovarian cancer early detection. Here, we present a protocol to collect fallopian tube cells from freshly received surgical specimens.
04:35
Obtaining Primary Ovarian Cancer Cells from Solid Specimens: A Method to Culture Epithelial Ovarian Cancer Cells from Ovarian Tumor Specimens
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2023
In this video, we describe a method for isolating and characterizing primary ovarian cancer cells from solid ovarian tumor tissue specimens. The epithelial cancer cells obtained using this protocol are suitable for understanding the processes that may lead to ovarian cancer initiation and development.
FAQs
Ovulation-associated injury can activate a coordinated repair response involving epithelial cell proliferation, migration, and remodeling of interactions with the extracellular matrix. These processes help restore tissue organization after disruption rather than representing isolated cellular events. Studying their coordination can reveal how ovarian tissue maintains its surface architecture and how abnormal repair may contribute to epithelial transformation.
Mechanical disturbance and inflammation associated with ovulation provide signals that can alter epithelial behavior. Cells respond through changes in proliferation, movement, and matrix interactions, linking the physical environment with tissue repair. Reproducing these influences in engineered systems is important because models that omit them may not adequately represent the dynamic conditions surrounding ovarian tissue.
Extracellular matrix interactions help regulate how ovarian surface epithelial cells organize, migrate, and participate in repair. They also provide a framework for examining cell-matrix signaling, which can influence epithelial remodeling and transformation. Incorporating these interactions into bioengineered models therefore supports more informative studies of tissue organization and mechanisms associated with ovarian disorders.
Engineered culture platforms provide controlled model systems for examining ovarian surface epithelial organization, wound healing, and responses to relevant tissue signals. They can be designed to investigate how cells interact with their surrounding matrix and how those interactions change during repair or transformation. Such platforms help connect cellular behavior with broader ovarian tissue processes.
Three-dimensional models can represent ovarian surface epithelial organization in a context that is more physiologically relevant than a simplified culture arrangement. They are particularly useful for studying spatial relationships, cell-matrix signaling, and tissue remodeling together. In bioengineering research, this added context can improve investigation of reproductive biology and ovarian disorders.
These models can be used to examine how ovarian surface tissue repairs injury, maintains organization, and undergoes epithelial transformation. They also support investigation of disease development by linking cellular responses with mechanical, inflammatory, and extracellular matrix conditions. The resulting systems are relevant to reproductive biology, ovarian tissue engineering, and research on ovarian disorders.