Macrophage Uptake
Macrophage Uptake - Related Videos
04:57
Fixed Cell Efferocytosis Assay: A Method to Study Efferocytic Uptake of Apoptotic Cells by Macrophages Using Fluorescence Microscopy
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2025
In this video, we demonstrate the efferocytosis assay to study the clearance of apoptotic cells using confocal microscopy. Efferocytosis is a multi-step process where apoptotic cells are engulfed by phagocytes and degraded within vesicles called efferosomes. This protocol provides a method to visualize internalized and non-internalized portions of individual apoptotic cells and quantify efferocytosis.
04:04
Radiolabeled Amino Acid Uptake Assay to Quantify Cellular Uptake of Amino Acids
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2025
This video demonstrates an in vitro technique for estimating amino acid uptake by bone marrow-derived stromal cells. Na+-dependent amino acid transporters mediate the uptake of radiolabeled amino acids inside the cells, and the cellular uptake is estimated by measuring the radioactivity.
03:06
The Effect of Aspirin and Ibuprofen on the Phagocytic Ability of Macrophages
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2025
Source:Adepemi O. Ogundeji1, Nozethu Mjokane1, Maphori Maliehe1, Olihile M. Sebolai11Department of Microbiology and Biochemistry, University of the Free State.This video showcases a method to assess the impact of aspirin and ibuprofen, at their minimum inhibitory concentrations, on macrophage phagocytosis using pH-sensitive fluorescent dye-stained fungal cells. The drugs inhibit cyclooxygenase-2, decreasing prostaglandin formation and boosting macrophage phagocytosis, as observed through...
10:07
Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages
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Cited by 326 •
2013
The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways.
08:06
Study of Phagolysosome Biogenesis in Live Macrophages
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Cited by 6 •
2014
Phagocytic cells play a major role in the innate immune system by removing and eliminating invading microorganisms in their phagosomes. Phagosome ...
FAQs
Receptor-mediated recognition acts as the initiating control point for macrophage uptake. Before material enters the cell, macrophage receptors identify extracellular targets and trigger internalization. This early recognition step links the presence of pathogens, debris, particles, or soluble material to the cellular pathways that determine how the material is enclosed and subsequently handled.
These pathways internalize material through different cellular processes. Phagocytosis takes in particles, pathogens, or cellular debris, whereas macropinocytosis internalizes soluble material through larger vesicular compartments. Endocytosis provides another route for bringing material into the cell. Comparing these pathways helps explain how macrophages handle varied targets during immune defense and tissue maintenance.
After internalization, the material remains within vesicles that undergo maturation inside the macrophage. These vesicles can fuse with lysosomes, compartments associated with degradation. Depending on the cargo and cellular handling, the outcome may include breakdown of material, processing of its components, or antigen presentation, connecting uptake with downstream immune functions.
Intracellular fate determines whether internalized material is degraded, processed, or used for antigen presentation. These outcomes give macrophage uptake consequences beyond entry into the cell, because they can influence immune defense and the handling of cellular debris. In biology, this makes uptake relevant to both host protection and tissue maintenance.
Macrophage uptake assays evaluate how macrophages interact with external materials after exposure. The approach is useful for studying responses to biomaterials, nanoparticles, and drug-delivery systems. Such investigations can reveal whether these materials engage macrophages and help researchers examine cellular handling in the context of immune biology and therapeutic development.
In biomaterials and nanoparticle research, uptake studies examine the interaction between macrophages and the material being investigated. The same framework can be applied to drug-delivery systems to determine how immune cells handle them. These observations support evaluation of material behavior in relation to macrophage activity and immune responses.
Macrophage uptake research connects cellular internalization with host responses to infection, inflammation, and tissue injury. By examining how macrophages handle pathogens, debris, or other material, researchers can relate uptake pathways to broader biological responses. This context helps explain how macrophages contribute to defense and tissue maintenance during changing physiological conditions.