Murine Ovarian Surface
Murine Ovarian Surface - Related Videos
03:06
A Method to Induce Ocular Surface Inflammation in a Murine Model
0 Views •
2025
This video demonstrates a method to induce ocular surface inflammation and meibomian gland dysfunction in a murine model. Stimulation of the mouse's immune system using an immunogen and later restimulation with the same immunogen promotes ocular surface inflammation, which causes plugging of meibomian gland secretion through neutrophil-produced aggNETs and leads to dry eye disease.
03:40
Orthotopic Inoculation of Ovarian Cancer Cells into Murine Ovary: A Procedure to Establish an Ovarian Cancer Mouse Model to Study Peritoneal Dissemination of Cells
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2023
This video describes the protocol for creating a murine experimental model to study tumor development and peritoneal metastasis. This ovarian cancer model is generated by the orthotopic inoculation of human epithelial ovarian cancer cells into the ovaries of a female mouse.
10:58
Quantitation of Intra-peritoneal Ovarian Cancer Metastasis
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Cited by 15 •
2016
Ovarian cancer metastasis is characterized by numerous diffuse intra-peritoneal lesions, such that accurate visual quantitation of tumor burden is challenging. Herein we describe a method for in situ and ex vivo quantitation of metastatic tumor burden using red fluorescent protein (RFP)-labeled tumor cells and optical imaging.
04:35
Obtaining Primary Ovarian Cancer Cells from Solid Specimens: A Method to Culture Epithelial Ovarian Cancer Cells from Ovarian Tumor Specimens
0 Views •
2023
In this video, we describe a method for isolating and characterizing primary ovarian cancer cells from solid ovarian tumor tissue specimens. The epithelial cancer cells obtained using this protocol are suitable for understanding the processes that may lead to ovarian cancer initiation and development.
08:17
Murine Experimental Model of Original Tumor Development and Peritoneal Metastasis via Orthotopic Inoculation with Ovarian Carcinoma Cells
0 Views •
Cited by 4 •
2016
To clarify the multistep process of peritoneal dissemination of ovarian carcinoma, the current study presents a murine experimental model of original tumor development and peritoneal metastasis via orthotopic inoculation with these tumor cells.
FAQs
Ovulation produces localized tissue disruption that activates coordinated surface-cell responses. Cells proliferate to increase coverage, migrate across the injured region, and remodel the surface as repair proceeds. Studying these responses helps researchers examine how normal restoration differs from cellular behaviors that could allow abnormal cells to emerge, persist, or contribute to early tumor development.
The native ovarian environment preserves relationships between surface epithelium and surrounding stroma that are difficult to interpret in isolation. This context allows researchers to examine how epithelial changes occur alongside tissue remodeling and stromal interactions. Such observations can clarify processes associated with early tumor development and progression rather than focusing only on altered cells outside their tissue setting.
Genetic manipulation can be used to examine how selected cellular changes affect ovarian surface behavior, while lineage tracing follows cells and their descendants over time. Together, these approaches help connect injury-related repair, abnormal cell persistence, and tumor development. They are particularly useful for investigating whether observed cell populations contribute to progression within the native ovary.
Studies can combine genetic manipulation, lineage tracing, and microscopic analysis of ovarian tissue. These approaches examine surface-cell behavior within its native environment, including changes associated with injury, repair, remodeling, and abnormal development. Using complementary methods helps researchers relate cellular identity and behavior to tissue structure, stromal interactions, and patterns relevant to ovarian cancer.
Microscopic analysis can document how the surface responds after localized disruption and whether coverage is restored through proliferation, migration, and remodeling. It can also identify tissue-level changes associated with abnormal cells, surrounding stroma, and early tumor development. These observations provide structural evidence that complements genetic and lineage-based analyses of ovarian surface behavior.
The model links a normal repair process with cancer-related questions. Researchers can examine how the ovarian surface restores coverage after injury, then investigate circumstances in which abnormal cells emerge or persist instead. This comparison supports studies of mechanisms underlying epithelial ovarian cancer progression and may help identify processes relevant to prevention before advanced disease develops.