Neutral Lipid Measurement
Neutral Lipid Measurement - Related Videos
10:56
A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence
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Cited by 32 •
2014
A simple protocol to determine the neutral lipid content of algal cells using a Nile Red staining procedure is described. This time-saving technique offers an alternative to traditional gravimetric-based lipid quantification protocols. It has been designed for the specific application of monitoring bioprocess performance.
03:32
Nile Red Staining: A Technique to Detect and Quantify Intracellular Neutral Lipids in Algae
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2025
This video demonstrates a protocol for detecting and quantifying neutral lipids in algal cells by staining them with Nile red dye.
02:44
Oil Red O Staining: A Technique for Staining Neutral Lipids in Hepatocytes for Detecting Hepatic Steatosis In Vitro
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2025
In this video, we demonstrate in vitro staining of neutral lipids in hepatocytes to detect hepatic steatosis or excessive fat accumulation in liver. Excessive fat is accumulated in the cells in form of lipid droplets. Oil Red O is a lipid soluble dye that binds to intracellular neutral lipids in these lipid droplets. When observed under a light microscope, these intracellular lipid droplets appear intensely red in color.
04:05
An Immunofluorescence Method to Measure the Neutralization of Human Cytomegalovirus
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2025
Source:Matthew J Murray1, Gary R McLean2,3, Matthew B Reeves11Institute of Immunity and Transplantation, University College London.2Cellular and Molecular Immunology Research Centre, London Metropolitan University.3National Heart and Lung Institute, Imperial College London.This video demonstrates an immunofluorescence assay designed to measure the neutralization of Human Cytomegalovirus (HCMV). Virus-specific antibodies are mixed with the virus and incubated with human fibroblast cells. The...
05:33
A Microneutralization Assay to Measure Neutralizing Antibody Titers in Human Serum
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2025
This video demonstrates Microneutralization assays to measure neutralizing antibodies using Madin-Darby Canine Kidney cells overexpressing α2,6-linked sialic acid in human sera. This assay is often used for influenza human...
FAQs
The measurement relies on transferring target lipids into an organic phase. Because triglycerides and cholesteryl esters are nonpolar, their recovery from the sample provides a chemical basis for quantification. Extraction conditions influence how much material enters the measured fraction, so incomplete or variable recovery can affect the reported lipid abundance and comparisons between samples.
Chromatography separates lipid components before detection, allowing the analysis to distinguish parts of the neutral-lipid mixture rather than treating every component as one combined signal. This separation is especially useful when the research question concerns lipid composition, because it can provide chemically resolved information in addition to an overall abundance-related measurement.
Fluorescent dyes partition into hydrophobic lipid phases and produce a detectable signal related to the amount of neutral lipid present. The resulting readout supports abundance estimation without requiring the same separation step used in chromatography. This approach is useful when the goal is to monitor changes in lipid content across biological, chemical, or treatment conditions.
A general workflow begins with selecting a measurement approach suited to the sample and the desired information. The sample may then undergo organic-solvent extraction, chromatographic separation, or dye-based detection. The resulting signal or separated lipid information is interpreted as an abundance or composition measurement, enabling comparisons among samples, formulations, or experimental conditions.
Researchers apply this analysis to study lipid metabolism, evaluate formulation development, examine food samples, and assess cellular responses to environmental or pharmacological conditions. The appropriate method depends on whether the main objective is measuring overall neutral-lipid abundance, characterizing composition, or tracking changes associated with a biological or chemical condition.
Results can help characterize lipid composition, estimate neutral-lipid abundance, assess sample quality, and identify changes associated with metabolism or experimental treatment. In chemistry, these outcomes connect molecular properties such as nonpolarity and phase behavior with practical measurements. The data can therefore support comparisons across samples and help evaluate how conditions affect lipid content.