Blood Investigations

Blood investigations are laboratory tests that analyze blood cells, proteins, chemicals, and other components to assess health and detect disease. They typically involve collecting a blood sample, separating or preparing its components, and applying methods such as cell counting, biochemical analysis, coagulation testing, or immunoassays to identify measurable changes. In clinical practice, these investigations support diagnosis, help evaluate organ function, assess infection, anemia, inflammation, or bleeding disorders, and monitor responses to treatment. Interpreting results alongside symptoms and medical history enables clinicians to make informed decisions and track changes in a patient’s condition over time.

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JoVE EoE - Assay Techniques

Endotoxin Activity Assay: A Chemiluminescence-Based Bioassay for Investigating Endotoxin Activity in Blood Sample

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2025

This video demonstrates a rapid bioassay for investigating endotoxin activity in a whole blood sample via a chemiluminescence-based method. This assay is a rapid and sensitive biomarker test for early-stage diagnosis of endotoxin-mediated sepsis.

Controlled Microfluidic Environment for Dynamic Investigation of Red Blood Cell Aggregation

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Cited by 8 •

2015

The protocol described details an experimental procedure to quantify Red Blood Cell (RBC) aggregates under a controlled and constant shear rate, based on image processing techniques. The goal of this protocol is to relate RBC aggregate sizes to the corresponding shear rate in a controlled microfluidic environment.

Repeated Blood Collection for Blood Tests in Adult Zebrafish

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Cited by 87 •

2015

Repeated blood sampling is necessary and important in animal research. We developed a novel, non-lethal and reliable method for repeated blood collection from adult zebrafish, and applied this method to the study of blood biochemistry, including glucose metabolism.

Fluorescence Micropipette Aspiration Assay to Investigate Red Blood Cell Mechanosensing

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Cited by 1 •

2024

The exploration of cellular behavior under mechanical stress is pivotal for advances in cellular mechanics and mechanobiology. We introduce the Fluorescence Micropipette Aspiration (fMPA) technique, a novel method combining controlled mechanical stimulation with comprehensive analysis of intracellular signaling in single cells. This technique investigates new in-depth studies of live-cell mechanobiology.

Improved Method for the Preparation of a Human Cell-based, Contact Model of the Blood-Brain Barrier

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Cited by 16 •

2013

Establishment of human models of the blood-brain barrier (BBB) can benefit research into brain conditions associated with BBB failure. We describe here an improved technique for preparation of a contact BBB model, which permits coculturing of human astrocytes and brain endothelial cells on the opposite sides of a porous membrane.

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