Barley Leaf Sheath
Barley Leaf Sheath - Related Videos
10:08
Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
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Cited by 1 •
2017
Many developmentally important genes have cell- or tissue-specific expression patterns. This paper describes LM RNA-seq experiments to identify genes that are differentially expressed at the maize leaf blade-sheath boundary and in lg1-R mutants compared to wild-type. The experimental considerations discussed here apply to transcriptomic analyses of other developmental phenomena.
11:09
Relating Stomatal Conductance to Leaf Functional Traits
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Cited by 39 •
2015
Unraveling how physiology and morphology are linked allows a deeper understanding of mechanistic functioning of plant leaves. We present both a procedure to derive parameters of stomatal regulation from stomatal conductance measurements and correlations with traditional functional leaf traits.
08:14
LeafJ: An ImageJ Plugin for Semi-automated Leaf Shape Measurement
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Cited by 93 •
2013
Demonstration of key methods for high throughput leaf measurements. These methods can be used to accelerate leaf phenotyping when studying many plant mutants or otherwise screening plants by leaf phenotype.
07:03
Assessing Stomatal Response to Live Bacterial Cells using Whole Leaf Imaging
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Cited by 38 •
2010
We have developed a simple and reproducible protocol to access stomatal response to live bacteria. This method minimizes wounding and manipulation of the leaf as compared to the use of epidermal peels reported previously.
08:15
Metabolomic Analysis of Barley by Gas Chromatography/Mass Spectrometry
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Cited by 1 •
2024
A method for metabolomic analysis of barley is presented. The method entails fractionation and derivatization of metabolites and analysis thereof by gas chromatography/mass spectrometry (GC/MS). Metabolomic analysis can be used to determine the effect of intercropping and drought stress on the grain.
FAQs
These processes act in sequence and coordination rather than independently. Cell division supplies new cells in the developing leaf primordium, cell expansion increases tissue length and extent, and differentiation establishes specialized sheath tissues. Their coordination determines how the sheath extends around successive stem regions and supports the developing architecture of the leaf and stem.
Vascular strands form as the sheath tissues develop and become part of the emerging tissue pattern. Their formation accompanies sheath extension around the stem, linking internal organization with the changing shape of the developing leaf base. Examining their timing and arrangement can therefore help relate tissue patterning to overall monocot leaf architecture.
Hormonal and environmental signals regulate the cellular processes that shape the sheath, including division, expansion, differentiation, and tissue patterning. Changes in these signals can modify how rapidly the sheath extends or how its tissues become organized. Studying these influences helps connect external conditions and internal regulation with visible differences in plant growth and structure.
A useful analysis follows the tissue from the leaf primordium through successive stages of sheath formation. Researchers can examine when cells proliferate, when they elongate, how they differentiate, and when vascular strands appear as the sheath extends around the stem. Comparing these stages reveals how cellular events are coordinated during monocot leaf development.
The sheath provides a system for connecting cellular behavior with larger plant form. Its development brings together cell division, expansion, differentiation, vascular formation, and tissue patterning in a clearly related leaf structure. This makes it useful for investigating how monocot leaves form and how developmental regulation contributes to differences in plant architecture and productivity.
Analysis can relate differences in sheath extension and tissue organization to underlying changes in cellular activity. Variations in proliferation, elongation, differentiation, vascular formation, or their regulation may produce differences in plant structure and growth. In a developmental biology context, this provides a cellular basis for examining how leaf and stem architecture may influence productivity.