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Immunology and Infection
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Assessing Stomatal Response to Live Bacterial Cells using Whole Leaf Imaging
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Assessing Stomatal Response to Live Bacterial Cells using Whole Leaf Imaging

Assessing Stomatal Response to Live Bacterial Cells using Whole Leaf Imaging

DOI:
10.3791/2185-v

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07:03 min

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October 02, 2010

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1Biology,University of Texas at Arlington

Chapters

  • 00:00Title
  • 00:50Introduction
  • 01:08Growing Plants and Preparing Bacteria
  • 02:58Leaf Staining and Incubation with Bacteria
  • 04:34Microscopy, Measurement, and Data Analysis
  • 05:21Representative Images
  • 06:43Conclusion

Summary

Automatic Translation

Automatic Translation

We have developed a simple and reproducible protocol to access stomatal response to live bacteria. This method minimizes wounding and manipulation of the leaf as compared to the use of epidermal peels reported previously.

Tags

Stomatal ResponseLive Bacterial CellsWhole Leaf ImagingGuard CellsStomatal PoreGas ExchangeLight IntensityCarbon Dioxide ConcentrationRelative HumidityPathogen EntryDisease DevelopmentArabidopsis PlantsPlant Innate ImmunityEpidermal PeelsEnvironmental ConditionsMES BufferElectrophysiological ExperimentsBacterial Suspension

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