Oocyte Microinjection
Oocyte Microinjection - Related Videos
12:10
Microinjection of Xenopus Laevis Oocytes
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Cited by 14 •
2009
Here we demonstrate cytoplasmic microinjection of Xenopus laevis oocytes with a nuclear import substrate, as well as preparation of the injected oocytes for visualization by thin-sectioning electron microscopy.
07:03
Mouse Oocyte Microinjection, Maturation and Ploidy Assessment
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Cited by 75 •
2011
Oocytes are prone to aneuploidy due to errors in chromosome segregation during meiotic maturation. Aneuploid eggs can cause infertility, miscarriages or developmental disorders like Down syndrome. Here, we describe methods to introduce materials of choice into oocytes and methods to study meiotic maturation and assess ploidy.
10:19
Generation of Genetically Modified Mice through the Microinjection of Oocytes
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Cited by 19 •
2017
The microinjection of mouse oocytes is commonly used for both classic transgenesis (i.e., the random integration of transgenes) and CRISPR-mediated gene targeting. This protocol reviews the latest developments in microinjection, with a particular emphasis on quality control and genotyping strategies.
07:06
Visualizing RNA Localization in Xenopus Oocytes
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Cited by 5 •
2010
Visualization of in vivo RNA transport is accomplished by microinjection of fluorescently labeled RNA transcripts into Xenopus oocytes, followed by confocal microscopy.
07:24
Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments
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Cited by 5 •
2016
Optimized procedures for the isolation of single follicles, cytoplasmic RNA microinjections, the removal of surrounding cell layers, and protein expression in Xenopus oocytes are described. In addition, a simple method for fast solution changes in electrophysiological experiments with ligand-gated ion channels is presented.
FAQs
Where the cargo is released matters because cytoplasmic and nuclear delivery place materials in different cellular contexts. Directing nucleic acids, proteins, or compounds to one of these compartments helps researchers examine how their location relates to early developmental processes. This choice connects the introduced material with specific molecular requirements during fertilization, cell division, or embryogenesis.
Microinjection at a defined developmental stage allows the introduced material to be linked to events occurring after delivery, rather than treating development as a single undifferentiated period. Researchers can therefore test relationships between a molecule and subsequent cell division, pattern formation, or embryogenesis. Stage control makes the timing of exposure part of the experimental design.
The fine glass needle and micromanipulator support controlled delivery while the procedure aims to minimize cellular damage. Precise amounts help distinguish effects associated with the selected nucleic acid, protein, compound, or other material from effects caused by inconsistent handling. If delivery is not controlled, developmental outcomes become harder to connect confidently to the material under investigation.
Researchers position an oocyte, guide a fine glass needle with a micromanipulator, penetrate the membrane, and release a selected cargo into the cytoplasm or nucleus. The workflow requires coordination between needle placement, controlled release, and protection of the cell from unnecessary injury. These steps establish the defined delivery conditions needed for developmental analysis.
It can be used to test gene function, alter maternal factors, introduce genome-editing reagents, and investigate molecular requirements for fertilization and embryogenesis. These applications allow researchers to associate a defined molecular intervention with early developmental changes. The method is especially useful when they need to examine how introduced components influence processes that begin soon after delivery.
Observed consequences may be examined in relation to cell division, pattern formation, fertilization, and embryogenesis. Because delivery occurs at a selected stage and involves a specified material, researchers can connect subsequent developmental outcomes to the intervention within a controlled experiment. This supports investigations of the molecular requirements underlying early developmental progression.