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Chapters
- 00:05Title
- 01:00Mouse Oocyte Collection
- 02:28Oocyte Microinjection and Maturation
- 04:14Ploidy Analysis
- 06:04A Euploid Metaphase II Egg
- 06:27Conclusion
Oocytes are prone to aneuploidy due to errors in chromosome segregation during meiotic maturation. Aneuploid eggs can cause infertility, miscarriages or developmental disorders like Down syndrome. Here, we describe methods to introduce materials of choice into oocytes and methods to study meiotic maturation and assess ploidy.
Tags
Mouse Oocyte MicroinjectionMaturationPloidy AssessmentMistakes In Chromosome SegregationAneuploid CellsSomatic CellsAneuploidyCancerGametesInfertilityMiscarriagesDevelopmental DisordersDown SyndromeHaploid GametesMeiosisMeiotic DivisionSister ChromatidsHomologous ChromosomesReductional DivisionErrors In Meiosis IIErrors In Male Meiosis 1Oocytes Arrest At Prophase Of MIGerminal Vesicle (GV)Ovulatory CuesAsymmetrical Cell DivisionMetaphase Of MIIFertilization By SpermIn Vitro Culture ConditionsTransgenic Mouse MutantsGene-targeted Mouse Mutants
