Mouse Oocyte Microinjection, Maturation and Ploidy Assessment

Paula Stein; Karen Schindler

doi:10.3791/2851

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Biology

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Mouse Oocyte Microinjection, Maturation and Ploidy Assessment

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Mouse Oocyte Microinjection, Maturation and Ploidy Assessment

Article DOI: 10.3791/2851

July 23rd, 2011 •

Paula Stein¹, Karen Schindler¹

¹Department of Biology, University of Pennsylvania

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July 23rd, 2011

Oocytes are prone to aneuploidy due to errors in chromosome segregation during meiotic maturation. Aneuploid eggs can cause infertility, miscarriages or developmental disorders like Down syndrome. Here, we describe methods to introduce materials of choice into oocytes and methods to study meiotic maturation and assess ploidy.

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