Zebrafish Cardiac Imaging
Zebrafish Cardiac Imaging - Related Videos
04:11
Functional Cardiac Imaging in Zebrafish Embryos Using Standard Microscopy and Video Analysis: Applications in Environmental and Biomedical Research
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2025
This protocol describes a low-cost light microscopy method to assess cardiac morphology and function in zebrafish embryos, enabling reproducible evaluation of developmental cardiotoxicity without the need for advanced imaging systems.
07:07
4D Light-sheet Imaging of Zebrafish Cardiac Contraction
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Cited by 8 •
2024
This protocol utilizes light-sheet imaging to investigate cardiac contractile function in zebrafish larvae and gain insights into cardiac mechanics through cell tracking and interactive analysis.
11:37
Cardiac Magnetic Resonance Imaging
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2023
Source: Frederick W. Damen and Craig J. Goergen, Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana In this video, high field, small-bore magnetic resonance imaging (MRI) with physiological monitoring is demonstrated to acquire gated cine loops of the murine cardiovascular system. This procedure provides a basis for assessing left-ventricular function, visualizing vascular networks, and quantifying motion of organs due to respiration. Comparable small animal...
04:58
Heart Dissection: A Method to Observe Zebrafish Cardiac Development
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2023
This video describes a technique of isolating the heart from a zebrafish at multiple developmental stages to study cardiac development, maturation, and ageing.
09:14
Cardiac Magnetic Resonance Imaging at 7 Tesla
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Cited by 10 •
2019
The sensitivity gain inherent to ultrahigh field magnetic resonance holds promise for high spatial resolution imaging of the heart. Here, we describe a protocol customized for functional cardiovascular magnetic resonance (CMR) at 7 Tesla using an advanced multi-channel radio-frequency coil, magnetic field shimming and a triggering concept.
FAQs
Fluorescent markers make selected cardiac cells visible during development, allowing investigators to follow their location and behavior as the heart forms. This cellular resolution helps relate individual cell movements to larger structural changes, including heart tube formation. In developmental biology, the approach provides a visual link between cardiac cell dynamics and the organization of the developing organ.
Live microscopy captures cardiac development over time rather than limiting analysis to one fixed stage. Researchers can observe changing heart structure, cardiac movement, and blood flow as development proceeds. This time-resolved view helps distinguish developmental events and connects structural formation with functional activity, making it useful for analyzing how the embryonic heart changes dynamically.
Imaging provides visible developmental outcomes that can be compared with the activity of genes and signaling pathways involved in cardiogenesis. Researchers can examine how altered regulation corresponds to changes in cardiac cells, heart tube formation, movement, or blood flow. This connection helps translate molecular mechanisms into observable effects at the level of the developing vertebrate heart.
A basic workflow uses zebrafish embryos, live microscopy, and fluorescent markers, followed by analysis of cardiac structure and function. Imaging can track cardiac cells and heart tube formation while also recording cardiac movement and blood flow. Together, these components provide complementary structural, cellular, and functional information from the same developmental model.
Analysis can focus on several observable outcomes: the arrangement of cardiac cells, progression of heart tube formation, cardiac movement, and blood flow. Considering these features together helps researchers evaluate both morphogenesis and function. Changes in any of them can indicate that cardiac development or regulation has been affected, providing a basis for comparing normal and altered conditions.
The same imaging strategy can be applied to different developmental and biomedical questions. It can reveal structural or functional abnormalities associated with congenital heart defects, follow cardiac regeneration, and show how the heart responds to drugs. Because embryos are optically accessible, researchers can examine these outcomes through visible changes in cells, structure, movement, or blood flow.