Site Directed Excision
Site Directed Excision - Related Videos
01:54
Base Excision Repair
0 Views •
2020
One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template. The first step of...
01:54
Base Excision Repair
0 Views •
2023
One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template. The first step of...
01:46
Nucleotide Excision Repair
0 Views •
2020
Exposure to mutagens can damage DNA and result in bulky lesions that distort the double-helix structure or impede proper transcription. Damaged DNA can be detected and repaired in a process called nucleotide excision repair (NER). NER employs a set of specialized proteins that first scan DNA to detect a damaged region. Next, NER proteins separate the strands and excise the damaged area. Finally, they coordinate the replacement with new, matching nucleotides.DNA distortion and damageCells are...
01:38
Nucleotide Excision Repair
0 Views •
2023
DNA Distortion and Damage Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
01:46
Nucleotide Excision Repair
0 Views •
2026
Exposure to mutagens can damage DNA and result in bulky lesions that distort the double-helix structure or impede proper transcription. Damaged DNA can be detected and repaired in a process called nucleotide excision repair (NER). NER employs a set of specialized proteins that first scan DNA to detect a damaged region. Next, NER proteins separate the strands and excise the damaged area. Finally, they coordinate the replacement with new, matching nucleotides.DNA distortion and damageCells are...
FAQs
Precision comes from placing compatible recognition sites on both sides of the DNA segment intended for removal. The site-specific recombinase binds these paired sequences and catalyzes recombination between them, restricting the reaction to the selected boundaries. This arrangement helps minimize unintended changes elsewhere in the chromosome or other DNA molecule.
The locations of the recognition sites determine the boundaries of the excised segment. When those sites flank a marker gene, regulatory element, or engineered sequence, recombination separates the intervening DNA from the surrounding molecule. Thus, target-site placement is the key design feature linking the intended genetic change to the resulting excision.
Excision does not simply remove DNA without a trace; it leaves a defined genetic junction at the recombined site. That predictable junction helps distinguish the intended genetic configuration from unintended alterations and supports cleaner system design. In experimental settings, the junction represents the remaining genomic arrangement after the selected sequence has been removed.
A researcher first identifies the DNA segment to be removed and positions paired recognition sites so that the desired sequence lies between them. The corresponding site-specific recombinase must then act on those sites to release the intervening DNA. This design connects the selected target, the recombination boundaries, and the intended final genetic arrangement.
In infection research, controlled removal can eliminate selected marker genes, regulatory elements, or engineered sequences from microbial genomes. Comparing systems before and after removal can help investigators focus on the consequences of the remaining genetic configuration while limiting unrelated alterations. This makes the approach relevant to examining pathogen biology with more precisely designed genetic systems.
Immune-cell engineering may require removal of marker genes or other engineered sequences after they have served their purpose. Site-directed excision provides a way to eliminate such defined elements while preserving a predictable genetic junction and minimizing unintended changes elsewhere. The resulting cleaner configuration can support research on engineered immune cells and their genetic design.