Donor Lung Harvest
Donor Lung Harvest - Related Videos
04:25
Harvesting Articular Cartilage From Joints of an Equine Donor: A Surgical Procedure to Isolate Vascular Connective Tissue From Cadaveric Joints of an Equine Donor
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2025
In this video, we demonstrate the extraction of articular cartilage from an equine cadaveric joint. The isolated articular cartilage can be used for further downstream analysis.
07:28
Donor Posterior Atrial Flap Rotation for Left Atrial Cuff Reconstruction in Lung Transplantation
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2024
This protocol describes a new method for effectively reconstructing donor atrial cuff anterior wall defects during lung transplantation through the rotation of the donor posterior atrial flap.
12:29
Sterile Tissue Harvest
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2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN In 1959 The 3 R's were introduced by W.M.S. Russell and R.L. Burch in their book The Principles of Humane Experimental Technique. The 3 R's are replacement, reduction, and refinement of the use of animals in research. The use of cell lines and tissue cultures that originated from research animals is a replacement technique, as it allows for many experiments to be conducted in vitro. Harvesting...
05:49
Establishment of an Ex Vivo Lung Perfusion Rat Model for Translational Insights in Lung Transplantation
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Cited by 5 •
2023
Here, we describe the necessary steps for establishing a rat EVLP model and show the inflammatory profile associated with the perfused lungs. The aim is to propagate knowledge and experiences about the rat EVLP model, enabling the integral understanding of the biological responses associated with that revolutionary technique.
21:15
Diagnostic Necropsy and Tissue Harvest
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2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN Many animal experiments rely on final data collection time points that are gathered from the harvesting and testing of organs and tissues. The use of appropriate methods for the collection of organs and tissues can impact the quality of the samples and the analysis of the data that is gleaned for the testing of the tissues. The method of euthanasia of the animal can also impact the quality of...
FAQs
Lung tissue remains vulnerable when blood supply is interrupted, so preservation focuses on reducing injury during the interval between retrieval and implantation. Cooling and flushing with a cold preservation solution lower metabolic activity and help protect graft quality. Effective control of this interval supports the possibility that the lungs will remain viable for transplantation.
The solution is flushed through the lungs after the airway and pulmonary vessels are divided. Its cold temperature reduces tissue metabolism, while the flush helps prepare the organs for preservation after retrieval. This step is central to limiting preservation-related damage before the lungs are cooled, packaged, transported, and implanted.
Surgeons carefully mobilize the lungs and then divide the airway and pulmonary vessels as part of retrieval. Managing these structures allows the organs to be separated for preservation while maintaining an organized transition from surgical removal to cold flushing. Precision during this stage contributes to graft quality and supports later transplantation.
Donor assessment and stabilization occur before the surgical retrieval begins. These steps help the procurement team establish suitable conditions for preserving the lungs and coordinating the operation. By addressing the donor first, the teams create a controlled pathway toward mobilization, vessel and airway division, flushing, cooling, and preparation for transport.
After cold flushing, the lungs are cooled further, packaged, and transported for implantation in the recipient. Packaging and transport extend the preservation workflow beyond the operating procedure, making careful handling and timing important to maintaining viability. The sequence connects retrieval with the recipient operation while limiting preservation-related damage.
The procedure makes donor lungs available for transplantation in patients with advanced respiratory disease. Its clinical value depends not only on retrieval, but also on preserving graft quality through stabilization, cold flushing, cooling, packaging, and transport. Coordination among procurement, critical care, and transplant teams helps connect a deceased donor with a recipient safely and efficiently.