Lactate Extraction Ratio
Lactate Extraction Ratio - Related Videos
03:08
Colorimetric Assay for Quantification of Lactate in Test Samples
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2025
This video describes a colorimetric assay for lactate measurement in cell lysates by using the lactate dehydrogenase enzyme. This enzyme converts lactate into pyruvate, along with the conversion of NAD to NADH that reacts with tetrazolium dyes and gives a colored product that can be measured spectrophotometrically to quantitate the lactate.
07:30
Using Chronic Social Stress to Model Postpartum Depression in Lactating Rodents
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Cited by 37 •
2013
This article describes the use of chronic resident intruder social stress as an ethologically relevant paradigm to model postpartum depression and anxiety in lactating rodents.
02:58
Evaluating Cytotoxicity and Membrane Integrity in Lung Slices via Lactate Dehydrogenase Assay
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2025
This video demonstrates the lactate dehydrogenase, LDH assay to measure the cytotoxic effect of a test substance and its effect on the membrane integrity in human precision-cut lung slices. The assay provides a quantitative measure of cell death or damage in the sample.
03:02
A Lactate Dehydrogenase Release Assay To Detect Macrophage Death Following Nigericin Exposure
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2025
This video demonstrates the lactate dehydrogenase assay to detect inflammasome-mediated macrophage death. The macrophages are exposed to lipopolysaccharide for priming, followed by exposure to nigericin to cause inflammasome-induced cell death, which is determined by the lactate dehydrogenase assay.
03:25
A Lactate Dehydrogenase Release Assay to Detect Cryptococcus neoformans Infection in Macrophages
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2025
In this video, we demonstrate a cytotoxicity assay to assess Cryptococcus neoformans, a pathogenic fungus, infection in macrophages. The cytotoxicity is determined by the release of cytoplasmic lactate dehydrogenase enzyme due to macrophage lysis caused by Cryptococcus neoformans infection.
FAQs
The sign identifies the direction of regional lactate exchange. A positive value reflects net removal from blood by the examined tissue, while a negative value reflects net release into the circulation. This distinction helps separate tissues acting as lactate consumers from those contributing lactate, especially when regional metabolism changes during illness or physiological stress.
The ratio describes the concentration change across a tissue, but it does not independently describe how much blood passes through that tissue. Interpreting the value alongside blood flow helps place the arteriovenous difference in physiological context. The combined information can clarify whether regional lactate handling reflects meaningful uptake or release under the measured conditions.
An elevated circulating lactate concentration alone cannot identify its underlying cause. Regional extraction findings provide additional context by showing whether a tissue is removing lactate or releasing it. When the ratio is interpreted with arterial and regional lactate concentrations, clinicians and researchers can better assess whether increased production, reduced clearance, or both may contribute to the observed pattern.
The calculation requires lactate concentrations on the arterial side and after blood has passed through the tissue or organ, allowing the arteriovenous concentration difference to be determined. That difference is then related to the arterial concentration and expressed as a percentage. Regional blood-flow information adds context when the result is interpreted.
This measure is useful when investigators need to characterize regional lactate metabolism rather than rely only on a circulating lactate value. It can support assessment of tissue perfusion, organ function, and metabolic responses during illness or physiological stress. Its value is greatest when regional exchange is examined together with concentration and blood-flow measurements.
Regional exchange patterns can show whether an organ or tissue is behaving primarily as a site of lactate uptake or release under the tested condition. Changes in that balance may provide information about metabolic adaptation, perfusion, or altered clearance. In medicine, these findings help connect systemic lactate abnormalities with the behavior of specific tissues or organs.