Field Excitatory Post-synaptic Potential
Field Excitatory Post-synaptic Potential - Related Videos
05:26
Recording Neuronal Field Excitatory Postsynaptic Potentials in Acute Hippocampal Slices
0 Views •
2025
This video demonstrates the procedure for setting up a brain slice in a submersion chamber to record synaptic activity. The process involves securing the slice on lens paper, maintaining a continuous flow of artificial cerebrospinal fluid (aCSF) to ensure neuronal viability, and accurately positioning the stimulation and recording electrodes relative to a specific brain region of interest. This setup allows for precise monitoring of baseline synaptic activity and the effects of electrical...
05:46
Electrophysiological Methods for Recording Synaptic Potentials from the NMJ of Drosophila Larvae
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Cited by 29 •
2009
Here we describe electrophysiological methods for measuring synaptic transmission at the neuromuscular junction of Drosophila larva. Evoked release is initiated artificially by stimulating the motor neuron axons, and transmission through the NMJ can be measured by the postsynaptic response evoked in the muscle.
09:12
Channelrhodopsin2 Mediated Stimulation of Synaptic Potentials at Drosophila Neuromuscular Junctions
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Cited by 24 •
2009
This procedure uses a blue light-activated algal channel and cell-specific genetic expression tools to evoke synaptic potentials with light pulses at the neuromuscular junction (NMJ) in Drosophila larvae. This technique is an inexpensive and easy-to-use alternative to suction electrode stimulation for synaptic physiology studies in research and teaching laboratories.
04:27
MicroRNA-Mediated Inhibition of Excitatory Postsynaptic Currents in Mouse Hippocampal Slices
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2025
This video demonstrates microRNA-mediated inhibition of excitatory synaptic currents in mouse hippocampal brain slices. The CA3 neurons were infected with a recombinant viral vector harboring a construct for expressing a fluorescent reporter, a light-activated membrane channel, and a microRNA that downregulates the expression of voltage-gated calcium channels. Upon placing a slice in a recording chamber and patching a CA1 neuron with a recording pipette, light pulses were used to stimulate the...
08:13
Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures
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Cited by 2 •
2013
This video demonstrates the procedures used to grow primary cultures of embryonic Xenopus nerve and muscle cells and the usefulness of this preparation for making simultaneous pre- and post-synaptic patch clamp recordings.
FAQs
The fEPSP slope is commonly treated as an estimate of synaptic strength, while amplitude provides another measure of the recorded population response. Examining these features after afferent-pathway stimulation allows investigators to quantify changes in excitatory transmission rather than relying only on whether a response is present. This supports analysis of synaptic modulation over time.
Synchronized activity makes excitatory currents from multiple neurons sufficiently coordinated to produce a measurable local voltage change. These currents create local current sinks, which the extracellular electrode detects as a population response. The resulting signal therefore reflects organized circuit activity rather than isolated synaptic events, helping researchers evaluate communication across an activated pathway.
Glutamate-mediated excitatory currents can contribute to the local current sinks generated when an afferent pathway is activated. Their synchronized activity helps shape the extracellular response recorded by the electrode. Consequently, changes in the measured signal can provide information about excitatory synaptic transmission and how that transmission is altered during experimental manipulation.
A typical workflow begins by stimulating an afferent pathway in a brain slice or intact preparation. An extracellular electrode then records the resulting population voltage change. Researchers analyze the response, commonly emphasizing its slope or amplitude, to estimate excitatory synaptic strength and compare transmission under different experimental conditions.
Researchers compare fEPSP measurements before and after conditions that produce long-term potentiation or long-term depression. Changes in slope or amplitude indicate whether excitatory synaptic transmission has strengthened or weakened at the population level. This approach connects lasting changes in recorded circuit responses with broader studies of neural plasticity.
Field Excitatory Post-synaptic Potential measurements can evaluate synaptic transmission, neural plasticity, and the effects of pharmacological interventions. They also support investigations of circuit mechanisms related to learning, memory, and neurological disease. Because the recordings summarize activity across many neurons, they help link cellular synaptic changes to circuit-level outcomes.