Micro Syringe Injection
Micro Syringe Injection - Related Videos
09:58
Syringe-injectable Mesh Electronics for Stable Chronic Rodent Electrophysiology
0 Views •
Cited by 31 •
2018
Mesh electronics probes seamlessly integrate and provide stable, long-term, single-neuron level recording within the brain. This protocol uses mesh electronics for in vivo experiments, involving the fabrication of mesh electronics, loading into needles, stereotaxic injection, input/output interfacing, recording experiments, and histology of tissue containing mesh probes.
07:52
Simultaneous Electrophysiological Recording and Micro-injections of Inhibitory Agents in the Rodent Brain
0 Views •
Cited by 3 •
2015
Here, we craft a glass pipette with dual functions: inhibition of deep brain structures by microinjections of drugs and real-time monitoring of their effects through simultaneous electrophysiological recordings.
07:53
Micro-particle Image Velocimetry for Velocity Profile Measurements of Micro Blood Flows
0 Views •
Cited by 12 •
2013
Micro-particle image velocimetry (μPIV) is used to visualize paired images of micro particles seeded in blood flows which are cross-correlated to give an accurate velocity profile. Shear rate, maximum velocity, velocity profile shape, and flow rate, each of which has clinical applications, can be derived from these measurements.
22:38
Chemotactic Response of Marine Micro-Organisms to Micro-Scale Nutrient Layers
0 Views •
Cited by 4 •
2007
The fabrication of microfluidic channels and their implementation in experiments for studying the chemotactic foraging behaviour of marine microbes within a patchy nutrient seascape and the swimming behaviour of bacteria within shear flow are described.
02:28
Extraction of Apoplastic Bacteria Using a Syringe-Based Pressure Method
0 Views •
2025
Source: Rufián, J. S., et al, Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (2022)This video demonstrates a syringe-based pressure method for extracting apoplastic bacteria, which proliferate and form microcolonies within the apoplast, the intercellular space between plant cells. The method yields debris-free bacterial samples suitable for downstream single-cell analysis.
FAQs
Injection volume, dispensing rate, and anatomical site jointly influence how localized the delivered reagent remains. Larger volumes or poorly controlled dispensing can increase unwanted spread, while inaccurate placement can expose unintended tissue. Controlling these variables helps researchers associate an observed neural or behavioral effect with the intended brain region rather than with imprecise delivery.
A stereotaxic apparatus supports consistent positioning of the fine needle at a defined brain location. Manual positioning can also be used, but the apparatus provides a structured approach for targeting sites when spatial accuracy is especially important. This positioning control strengthens comparisons among animals or experimental conditions and supports more reliable interpretation of localized neural effects.
Drugs and tracers provide different experimental outcomes when delivered through the same injection approach. A drug can be used to manipulate activity or neurotransmission in a selected region, whereas a tracer can label an anatomical location or pathway. Choosing between them depends on whether the study focuses on functional effects, neural connectivity, or both.
A typical workflow includes attaching a fine needle to a microsyringe, positioning it manually or with a stereotaxic apparatus, and moving the plunger in a controlled manner to dispense the selected reagent. The operator must coordinate the target site, injection volume, and dispensing rate so the delivered material remains appropriately localized for the experimental question.
Researchers use this approach when an experiment requires manipulation or labeling of a defined brain region rather than broad exposure. It can support investigations of neural circuits, neurotransmission, and behavior by placing drugs, tracers, or other reagents at selected sites. The resulting localization helps connect a regional intervention with physiological or behavioral observations.
Localized delivery can reduce uncertainty about which tissue received the experimental reagent. When the site, volume, and dispensing rate are carefully controlled, observed changes in neural activity, neurotransmission, or behavior can be interpreted more confidently as related to the intended region. This also improves reproducibility by making injection conditions more consistent across experiments.