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Dissection of Saccharomyces Cerevisiae Asci
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JoVE 신문 생물학
Dissection of Saccharomyces Cerevisiae Asci
DOI:

12:57 min

May 19, 2009

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Chapters

  • 00:00Title
  • 00:45Introduction
  • 01:15Construction of Diploid Strains
  • 03:01Sporulation and Digestion of Asci
  • 04:57Dissection of Asci
  • 08:56Relocation of Zygotes
  • 11:08Representative Results
  • 12:14Conclusion

Summary

자동 번역

Micromanipulation of yeast cells is needed for meiotic genetic analysis or to select diploid zygotes. These micromanipulations are carried out using the microneedle of a dissection microscope. The microneedle is used to relocate cells and is controlled by a micromanipulator which are available with various degrees of automation.

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