Experiment
JoVE Encyclopedia of Experiments
Encyclopedia of Experiments: Biology
Encyclopedia of Experiments: Biology
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Pyruvate Assay for Estimation of Pyruvate Levels in Cellular Fractions of Worms
Transcript
Use a colorimetric assay kit to measure the concentration of pyruvate in the test samples, and carry out duplex examinations.
Add 10 microliters of the test samples to different wells of a 96-well plate, and add 90 microliters of working reagent to each sample. Incubate covered for 30 minutes at room temperature. Then, place the plate in a microplate reader to measure the absorbance of each well at 570 nanometers.
Plot the pyruvate standard curve and calculate the pyruvate concentrations from the standard curve by multiplying each concentration by 2.25.
