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Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation

Article DOI: 10.3791/2244-v • 10:35 min • September 17th, 2010
September 17th, 2010 •
1, 1,2, 1,2, 1
1Neuroscience Center, University of Helsinki, 2Faculty of Biological and Enviromental Sciences, University of Helsinki

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This protocol describes a non-viral method of delivery of genetic constructs to a certain area of living rodent brain. The method consists of plasmid preparation, micropipette fabrication, neonatal rat pup surgery, microinjection of the construct, and in vivo electroporation.

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Gene Delivery Postnatal Rat Brain Non-ventricular Plasmid Injection Electroporation Transgenic Animals Gene Of Interest Knockout Animals Compensatory Mechanisms Gene Expression Timing Transfected Cells Microinjection In Vivo Electroporation Targeted Delivery SiRNA Dye Molecules Newborn Rodent Brain Non-migratory Cell Types Two-photon In Vivo Imaging Electrophysiological Experiments
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