Functional Imaging of Brown Fat in Mice with <sup>18</sup>F-FDG micro-PET/CT

Xukui Wang; Laurie J. Minze; Zheng-Zheng Shi

doi:10.3791/4060

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Biology

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Functional Imaging of Brown Fat in Mice with ¹⁸F-FDG micro-PET/CT

FDG micro-PET/CT와 마우스의 브라운 지방의 기능 영상

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10:53 min

November 23, 2012

DOI: 10.3791/4060-v

Xukui Wang¹, Laurie J. Minze², Zheng-Zheng Shi¹

¹Department of Translational Imaging,The Methodist Hospital Research Institute, Houston, ²Diabetes Research Center,The Methodist Hospital Research Institute, Houston

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Overview

This article describes a method for non-invasively assessing brown adipose tissue (BAT) activity in live mice using a standardized micro-PET/CT protocol. The technique involves cold-stimulation to induce BAT activity and measures the uptake of 18F-Fluorodeoxyglucose (FDG).

Key Study Components

Area of Science

Neuroscience
Metabolism
Imaging Techniques

Background

Brown adipose tissue plays a crucial role in thermogenesis and energy expenditure.
Understanding BAT activity can provide insights into metabolic disorders.
Non-invasive imaging techniques are essential for studying live animal models.
Micro-PET/CT offers a robust method for visualizing metabolic processes.

Purpose of Study

To measure BAT activity in live mice non-invasively.
To assess the effects of cold exposure on FDG uptake in BAT.
To establish a standardized imaging protocol for future studies.

Methods Used

Cold treatment of mice at 4°C for four hours to induce BAT activity.
Injection of 18F-FDG followed by an additional hour at 4°C.
Micro-PET and micro-CT imaging using a dedicated small animal imaging system.
Post-imaging processing to coregister and analyze PET and CT images.

Main Results

Successful non-invasive measurement of BAT activity in live mice.
Significant differences in FDG uptake were detected under cold-stimulated conditions.
The method demonstrated robustness and sensitivity for detecting BAT activity.
Findings contribute to understanding the role of BAT in energy metabolism.

Conclusions

The standardized micro-PET/CT protocol is effective for assessing BAT activity.
This method can be applied in various research contexts involving metabolism.
Further studies may explore the implications of BAT activity in metabolic health.

Frequently Asked Questions

What is the significance of brown adipose tissue?

Brown adipose tissue is important for thermogenesis and energy expenditure in mammals.

How does cold exposure affect BAT activity?

Cold exposure stimulates BAT activity, increasing its metabolic rate and FDG uptake.

What imaging techniques are used in this study?

The study uses micro-PET and micro-CT imaging techniques for non-invasive assessment.

What is 18F-FDG and why is it used?

18F-FDG is a radiotracer used to measure glucose metabolism in tissues, including BAT.

Can this method be applied to other research areas?

Yes, the method can be adapted for studies on metabolism and energy expenditure in various contexts.

마우스 갈색 지방 조직 (BAT)의 기능 영상의 방식은 BAT에서 18F-Fluorodeoxyglucose의 냉 자극 이해 (FDG)가 아닌 invasively 표준화 micro-PET/CT 프로토콜로 과세되는 설명되어 있습니다. 이 방법은 마우스 모델에서 BAT 활동의 차이를 감지 할 강력하고 민감하다.

이 절차의 전반적인 목표는 표준화된 마이크로 PET CT 프로토콜로 살아있는 마우스에서 비침습적으로 갈색 지방 조직 활성을 측정하는 것입니다. 이것은 먼저 동물을 섭씨 4도에서 4시간 동안 냉간 처리하여 갈색 지방 조직 활동을 유도함으로써 달성됩니다. 다음으로 18FTG의 불소를 주입하고 동물을 섭씨 4도에서 1시간 더 배양하여 활동 흡수를 달성합니다.

그런 다음 소형 동물 전용 이미징 시스템을 사용하여 마이크로 CT 및 마이크로 PET 이미징 시퀀스를 수행합니다. 마지막으로, 사후 이미징 처리 소프트웨어를 사용하여 획득한 애완 동물 이미지를 CT 이미지와 함께 정합한 다음 견갑간 박쥐 영역의 FDG 흡수를 분석하여 박쥐 활동을 나타냅니다. 갈색 지방 조직은 성체뿐만 아니라 뼈 포유류의 에너지 소비와 열 발생에 중요한 역할을 합니다.

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