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Journal / Biology / Isolation, Kultur og funktionel karakterisering…
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Isolation, Kultur og funktionel karakterisering af Voksen Mouse Cardiomyoctyes
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Isolation, Kultur og funktionel karakterisering af Voksen Mouse Cardiomyoctyes

Article DOI: 10.3791/50289-v • 12:49 min • September 24th, 2013
September 24th, 2013 •
1, 1,2, 1, 1, *1, *1
1Cardiovascular Institute, Beth Israel Deaconess Medical Center, Harvard Medical School, 2Division of Cardiology, Sapienza University
* These authors contributed equally

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Her beskrives isoleringen af ​​voksne mus cardiomyoctyes bruger Langendorff perfusion system. De resulterende celler er Ca2 +-tolerante elektrisk hvilende og kan dyrkes og transficeres med adeno-eller lentivirus at manipulere genekspression. Deres funktionalitet kan også analyseres ved hjælp af MMSYS systemet og patch clamp teknikker.

Tags

Cellebiologi medicin kardiologi cellebiologi anatomi fysiologi Mus ionkanaler Primær cellekultur hjertets elektrofysiologi voksen mus kardiomyocytter celleisolering IonOptix Cell Culture adenoviral transfektion patch clamp fluorescerende nanosensor
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