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Journal / Immunology and Infection / Isolierung von myeloider dendritischer Zellen und …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolierung von myeloider dendritischer Zellen und Epithelzellen Menschliche Thymus
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Isolierung von myeloider dendritischer Zellen und Epithelzellen Menschliche Thymus

Article DOI: 10.3791/50951-v • 09:07 min • September 19th, 2013
September 19th, 2013 •
1,2, 1, 3, 4, 5, 1
1Department of General Neurology, Hertie Institute for Clinical Brain Research, 2Institute of Pharmacology, University of Bern, 3Department of Immunology, University Medical Center Hamburg-Eppendorf, 4Department of Thoracic and Cardiovascular Surgery, University Clinic Tuebingen, 5Department of Neurology, University Hospital Erlangen

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Diese Protokollinformationen ein Verfahren zur Isolierung von Antigen-präsentierenden Zellen menschlichen Thymus über verschiedene Schritte der enzymatischen Verdauung des Gewebes, gefolgt von Dichtegradientenzentrifugation der Einzelzellsuspension und schließlich magnetisch und / oder FACS-Sortierung von Zellpopulationen von Interesse.

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Immunologie Immunsystem Prozesse die biologische Prozesse Immunologie Immunsystem-Krankheiten Immunsystem Phänomene Life Sciences (Allgemein) Immunologie menschlichen Thymus Isolation dendritische Zellen mTEC cTEC
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