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Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
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Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow

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10:33 min

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January 08, 2014

DOI:

10.3791/51023-v

DOI:
10.3791/51023-v

•

10:33 min
January 08, 2014

•

1 Views
, , , , , , , ,
1Department of Chemical and Biomolecular Engineering,Ohio University, 2Biomedical Engineering Program,Russ College of Engineering and Technology, Ohio University, 3Department of Dermatology,Brigham and Women’s Hospital, Harvard Medical School

Summary

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Compared with traditional affinity chromatography using protein A agarose bead-packed columns, protein A membrane adsorbers can significantly speed laboratory-scale isolation of antibodies and other Fc fragment-expressing proteins. Appropriate analysis and quantification methods can further accelerate protein processing, allowing isolation/characterization to be completed in one workday, instead of 20+ work hours.

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Affinity ChromatographyProtein AMembrane AdsorberFc-expressing ProteinsChimeric ProteinsStreamlined WorkflowProtein PurificationCell Culture SupernatantWestern BlottingFlow Cytometry

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Cite this Article

Burdick, M. M., Reynolds, N. M., Martin, E. W., Hawes, J. V., Carlson, G. E., Cuckler, C. M., Bates, M. C., Barthel, S. R., Dimitroff, C. J. Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow. J. Vis. Exp. (83), e51023, doi:10.3791/51023 (2014).

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