JoVE Journal
Medicine
Medicine
이 콘텐츠 오픈 액세스로 구독없이 시청하실 수 있습니다.
챕터
요약
Protocol for propagation of dissociated high grade glioma surgical specimens in serum-free neurosphere medium to select for cells with cancer stem cell phenotype. For specimens that fail to grow as neurospheres, an alternative protocol is suggested. A paraffin embedding technique for maintaining the 3D neurosphere architecture for immunocytochemistry is described.