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Immunology and Infection

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JoVE Journal Immunology and Infection
Mycket Lösta Intravital Randig-belysning Mikroskopi germinalcenter
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Mycket Lösta Intravital Randig-belysning Mikroskopi germinalcenter

Article DOI: 10.3791/51135-v • 10:07 min • April 9th, 2014
April 9th, 2014 •
*1,2, *3, 4, 2, *3,5, *1
1Biophysical Analytics, German Rheumatism Research Center, Leibniz Institute, 2Microscopy Core Facility, Max-Delbrück Center for Molecular Medicine, 3Immunodynamics, German Rheumatism Research Center, Leibniz Institute, 4LaVision Biotec GmbH, 5Immunodynamics and Intravital Imaging, Charité - University of Medicine
* These authors contributed equally

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Högupplösande intravital avbildning med förstärkt kontrast upp till 120 ^ m djup i lymfkörtlar från vuxna möss åstadkoms genom att rumsligt modulering av exciteringsmönster av en multi-fokal två-foton-mikroskop. I 100 um djup mätte vi resolutioner av 487 nm (lateral) och 551 nm (axial), på så sätt kringgå spridnings och diffraktion gränser.

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Immunologi två-foton-laserskanning mikroskopi deep-vävnad intravital avbildning germinal center lymfkörtel hög upplösning förbättrad kontrast
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