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JoVE Journal Medicine
Etablering af en
Evaluation of Vascular Control Mechanisms Utilizing Video Microscopy of Isolated Resistance Arteries of Rats
Journal (Medicine)
Evaluation of Vascular Control Mechanisms Utilizing Video Microscopy of Isolated Resistance Arteries of Rats
Assessment of Cardiac Morphological and Functional Changes in Mouse Model of Transverse Aortic Constriction by Echocardiographic Imaging
Journal (Medicine)
Assessment of Cardiac Morphological and Functional Changes in Mouse Model of Transverse Aortic Constriction by Echocardiographic Imaging
 
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Etablering af en

Article DOI: 10.3791/51278-v • 16:26 min • June 28th, 2014
June 28th, 2014 •
1, 1, 2, 2, 2
1VECT-HORUS SAS, 2Aix-Marseille Université, CNRS, NICN UMR 7259

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Formålet med den foreliggende undersøgelse var at validere reproducerbarheden af en in vitro BBB model involverer en rotte syngen co-kultur af endothelceller og astrocytter. Endotelcellen monolag præsenteret høj TEER og lav LY permeabilitet. Ekspression af specifikke TJ proteiner blev funktionelle reaktioner på betændelse og funktionalitet af transportører og receptorer vurderes.

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Medicine rottehjerne endotelceller (RBEC) muse rygmarv tight junction (TJ) receptor-medieret transport (RMT) low density lipoprotein (LDL) LDLR transferrin TFR P-glycoprotein (P -gp) transendothelial elektrisk modstand (TEER)
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