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Immunology and Infection

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A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses

Article DOI: 10.3791/51506-v • 14:23 min • August 31st, 2014
August 31st, 2014 •
*1, *1, 1,2
1Emory Vaccine Center at Yerkes National Primate Research Center, Emory University, 2Department of Pathology and Laboratory Medicine, Emory University
* These authors contributed equally

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요약
Automatic Translation

HIV-1 pathogenesis is defined by both viral characteristics and host genetic factors. Here we describe a robust method that allows for reproducible measurements to assess the impact of the gag gene sequence variation on the in vitro replication capacity of the virus.

Tags

Restriction Enzyme Based Cloning HIV-1 Subtype C Gag-MJ4 Chimeric Viruses In Vitro Replication Capacity HLA Class I Alleles Conserved Portions Of HIV-1 Genome Cellular Immune Pressure Transmission Of HLA-linked Polymorphisms Reduced Set Point Viral Loads Acute Time Points Subtype C Infected Zambians Proviral Backbone MJ4 Recombination Based Methods Replication Capacity Assessment
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