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Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells
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Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells

Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells

14,040 Views

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10:33 min

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May 22, 2014

DOI:

10.3791/51609-v

DOI:
10.3791/51609-v

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10:33 min
May 22, 2014

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38 Views
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1Developmental Biology, Center for Stem Cell Biology,Sloan-Kettering Institute for Cancer Research, 2St. Giles Laboratory of Human Genetics of Infectious Diseases,The Rockefeller University

Summary

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Neural crest (NC) cells derived from human pluripotent stem cells (hPSC) have great potential for modeling human development and disease and for cell replacement therapies. Here, a feeder-free adaptation of the currently widely used in vitro differentiation protocol for the derivation of NC cells from hPSCs is presented.

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Feeder-freeNeural Crest Progenitor CellsHuman Pluripotent Stem CellsIn Vitro DifferentiationEmbryonic DevelopmentPeripheral Nervous SystemMelanocytesMesenchymal CellsRegenerative MedicineDisease Modeling

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Cite this Article

Zeltner, N., Lafaille, F. G., Fattahi, F., Studer, L. Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells. J. Vis. Exp. (87), e51609, doi:10.3791/51609 (2014).

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