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Neuroscience

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JoVE Journal Neuroscience
Detección de
Intracranial Injection of Adeno-associated Viral Vectors
Journal (Neuroscience)
Intracranial Injection of Adeno-associated Viral Vectors
Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins
Journal (Neuroscience)
Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins
 
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Detección de

Article DOI: 10.3791/52139-v • 10:31 min • January 20th, 2015
January 20th, 2015 •
1, 1, 1, 2, 2, 2, 2, 1
1Department of Molecular Biology and Biochemistry, Simon Fraser University, 2Department of Biomedical Physiology and Kinesiology, Simon Fraser University

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Este protocolo demuestra cómo la proximidad Ligadura ensayo se puede usar para detectar in situ en las interacciones proteína-proteína en la unión neuromuscular Drosophila larval. Con esta técnica, los discos grandes y Hu-li shao tai se muestra para formar un complejo en la región postsináptica, una asociación identificado previamente a través de co-inmunoprecipitación.

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Neurociencia Número 95 adducin disección de la pared del cuerpo la biología del desarrollo discos grandes, Shao tai Hu-li inmunohistoquímica unión neuromuscular la neurociencia la interacción proteína-proteína proximidad ligadura de ensayo las larvas
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