Transfecting RAW264.7 Cells with a Luciferase Reporter Gene

Sylvia T. Cheung; Soroush Shakibakho; Eva Y. So; Alice L-F Mui

doi:10.3791/52807

Journal

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생물학

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루시퍼 라제 리포터 유전자 RAW264.7 세포를 형질

Transfecting RAW264.7 Cells with a Luciferase Reporter Gene

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Transfecting RAW264.7 Cells with a Luciferase Reporter Gene

루시퍼 라제 리포터 유전자 RAW264.7 세포를 형질

Please note that all translations are automatically generated. Click here for the English version.

20,593 Views

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12:58 min

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June 18, 2015

DOI:

10.3791/52807-v

DOI:

10.3791/52807-v

•

12:58 min

June 18, 2015

•

30 Views

Sylvia T. Cheung^1,2,3, Soroush Shakibakho^1,2, Eva Y. So^1,2, Alice L-F Mui^1,2,3

¹Immunity and Infection Research Centre,Vancouver Costal Health Research Institute, ²Department of Surgery,University of British Columbia, ³Department of Biochemistry and Molecular Biology,University of British Columbia

Summary

Transfection into the macrophage cell line, RAW264.7, is difficult due to the cell’s natural response against foreign materials. We described here a gentle yet robust procedure for transfecting luciferase reporter genes into RAW264.7 cells.

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루시퍼 라제 리포터 유전자 RAW264.7 세포를 형질

Summary

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