Isolation and Primary Culture of Mouse Aortic Endothelial Cells

Jie-Mei Wang; Alex F. Chen; Kezhong Zhang

doi:10.3791/52965

Journal

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생물학

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분리 및 마우스 대동맥 내피 세포의 차 문화

Isolation and Primary Culture of Mouse Aortic Endothelial Cells

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JoVE 신문 생물학

Isolation and Primary Culture of Mouse Aortic Endothelial Cells

분리 및 마우스 대동맥 내피 세포의 차 문화

Please note that all translations are automatically generated. Click here for the English version.

30,498 Views

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08:20 min

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December 19, 2016

DOI:

10.3791/52965-v

DOI:

10.3791/52965-v

•

08:20 min

December 19, 2016

•

50 Views

Jie-Mei Wang¹, Alex F. Chen², Kezhong Zhang¹

¹Center for Molecular Medicine and Genetics,Wayne State University, ²Third Xiangya Hospital and the Institute of Vascular Disease and Translational Medicine,Central South University

내레이션 대본

Summary

The vascular endothelial cells play a significant role in many important cardiovascular disorders. This article describes a simple method to isolate and expand endothelial cells from the mouse aorta without using any special equipment. Our protocol provides an effective means of identifying mechanisms in endothelial cell physiopathology.