Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress

Laura M. Ketelboeter; Sonia L. Bardy

doi:10.3791/53105

Journal

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면역학 및 감염병학

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방법은 세균 Pyomelanin 생산을 억제하고 산화 스트레스에 감도에 대응 증가를 확인하는 방법

Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress

방법은 세균 Pyomelanin 생산을 억제하고 산화 스트레스에 감도에 대응 증가를 확인하는 방법

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8,137 Views

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11:00 min

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August 31, 2015

DOI:

10.3791/53105-v

DOI:

10.3791/53105-v

•

11:00 min

August 31, 2015

•

2 Views

Laura M. Ketelboeter¹, Sonia L. Bardy¹

¹Department of Biological Sciences,University of Wisconsin Milwaukee

Summary

Bacterial pyomelanin production results in increased resistance to oxidative stress and virulence. We report on techniques that can be used to determine inhibition of pyomelanin production and assay the resulting increase in sensitivity to oxidative stress in bacteria, as well as determine antibiotic minimum inhibitory concentration (MIC).