심 초음파 이미징에 의해 가로 대동맥 수축의 마우스 모델에서 심장 형태 학적 평가 및 기능 변경

Overview

This protocol outlines a noninvasive method to assess cardiac structural and functional changes in a mouse model of heart disease induced by transverse aortic constriction (TAC). Utilizing echocardiographic imaging techniques, this approach enables researchers to monitor the progression of heart disease over time.

Key Study Components

Area of Science

• Cardiovascular research
• Mouse models of heart disease
• Echocardiography

Background

• Transverse aortic constriction (TAC) is a common method to induce heart disease in mice.
• Echocardiographic imaging provides a non-invasive means to evaluate cardiac function.
• Monitoring heart disease progression is crucial for understanding cardiovascular conditions.
• Challenges exist in acquiring accurate echocardiographic measurements without proper demonstration.

Purpose of Study

• To assess cardiac structure and function in a mouse model of heart disease.
• To provide a reliable method for monitoring heart disease progression.
• To address key questions in cardiovascular research through imaging techniques.

Methods Used

• Application of electrode gel to the paws of the mouse.
• Use of an electrode pad for measurement acquisition.
• Insertion of a rectal probe to monitor body temperature during imaging.
• Utilization of B- and M-mode echocardiography and Doppler imaging techniques.

Main Results

• Successful non-invasive assessment of cardiac structure and function.
• Reliable monitoring of heart disease progression over time.
• Demonstration of echocardiographic imaging effectiveness in a mouse model.
• Insights into the cardiovascular research field regarding heart disease.

Conclusions

• The echocardiographic imaging method is effective for assessing heart disease in mice.
• This protocol can aid in understanding the progression of cardiovascular conditions.
• Non-invasive techniques are crucial for longitudinal studies in heart disease research.

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