Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography

Andrii Ishchenko; Vadim Cherezov; Wei Liu

doi:10.3791/54463

Journal

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생화학

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준비 및 직렬 펨토초 결정학에 대한 지질 큐빅 단계에서 단백질 미세 배달

Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography

JoVE 신문
생화학

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JoVE 신문 생화학

Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography

준비 및 직렬 펨토초 결정학에 대한 지질 큐빅 단계에서 단백질 미세 배달

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11,376 Views

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09:09 min

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September 20, 2016

DOI:

10.3791/54463-v

DOI:

10.3791/54463-v

•

09:09 min

September 20, 2016

•

26 Views

Andrii Ishchenko^1,2, Vadim Cherezov^1,2, Wei Liu³

¹The Bridge Institute,University of Southern California, ²Department of Chemistry,University of Southern California, ³School of Molecular Sciences, Center for Applied Structural Discovery at the Biodesign Institute,Arizona State University

내레이션 대본

Summary

We describe procedures for the preparation and delivery of membrane protein microcrystals in lipidic cubic phase for serial crystallography at X-ray free-electron lasers and synchrotron sources. These protocols can also be applied for incorporation and delivery of soluble protein microcrystals, leading to substantially reduced sample consumption compared to liquid injection.