Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification

Dean A. Houston; Katherine A. Staines; Vicky E. MacRae; Colin Farquharson

doi:10.3791/54978

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쥐 배아 중족골의 문화 : 연골 골화의 생리 학적 모델

Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification

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Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification

쥐 배아 중족골의 문화 : 연골 골화의 생리 학적 모델

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11,053 Views

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07:23 min

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December 03, 2016

DOI:

10.3791/54978-v

DOI:

10.3791/54978-v

•

07:23 min

December 03, 2016

•

46 Views

Dean A. Houston*¹, Katherine A. Staines*¹, Vicky E. MacRae¹, Colin Farquharson¹

¹Developmental Biology, The Roslin Institute and R(D)SVS,The University of Edinburgh

내레이션 대본

Summary

We present a protocol to dissect and culture embryonic day 15 (E15) murine metatarsal bones. This highly physiological ex vivo model of endochondral ossification provides conditions closer to the in vivo situation than cells in monolayer or 3D culture and is a vital tool for investigating bone growth and development.