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Chapters
- 00:05Title
- 01:15Production of 15N, 13C-Tau
- 04:18Purification of 15N, 13C-Tau
- 07:38Acquisition of NMR Spectra
- 10:28Results: Evaluation of the Purification Procedure of the Recombinant Protein and NMR Spectrum
- 11:35Conclusion
We describe here a method to identify multiple phosphorylations of an intrinsically disordered protein by Nuclear Magnetic Resonance Spectroscopy (NMR), using Tau protein as a case study. Recombinant Tau is isotopically enriched and modified in vitro by a kinase prior to data acquisition and analysis.
Tags
Nuclear Magnetic Resonance SpectroscopyIntrinsically Disordered ProteinsTau ProteinPhosphorylationMolecular RegulationDiseaseProtein InteractionProtein StructureProtein FunctionAPtc VirusTherapyProtein InhibitorsBL21 CellsPlasmid DNALuria Bertani MediumAmpicillinM9 MediumIsotope LabelingRecombinant PlasmidOptical Density
