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Journal / Genetics / DNA kirlenme ribozomal DNA tabanlı RNA…
JoVE Journal
Genetics

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DNA kirlenme ribozomal DNA tabanlı RNA örneklerinde değerlendirilmesi

Article DOI: 10.3791/55451-v • 13:16 min • January 22nd, 2018
January 22nd, 2018 •
1, 1, 2, 3, 4
1Genetic and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU), 2Department of Industrial and Environmental Biotechnology, National Institute of Genetics Engineering and Biotechnology (NIGEB), 3Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences & Natural Resources, 4RG Abiotic Stress Genomics/ RG Heterosis, Department Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)

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Burada, RNA örneklerinde genomik DNA (gDNA) kirlilik izleme için bir iletişim kuralı mevcut. Sunulan yöntem astar özel ribozomal DNA (rDNA) gen iç kopya etmek spacer bölge (ITS) için kullanır. Yöntem güvenilir ve hassas algılama DNA kirlenme çoğu Ökaryotlar ve prokaryotlar için uygundur.

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Genetik sayı: 131 qPCR RT-qPCR gen ifade analizi DNA kirlenme tahlil ribozomal DNA (rDNA) ribozomal RNA (rRNA) buğdaygiller prokaryotlar ökaryot evrensel astar
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