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Cancer Research

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JoVE Journal Cancer Research
Genomweiten RNAi-Screening, Host Faktoren zu identifizieren, die onkolytische Viren Therapie zu modulieren
An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis
Journal (Cancer Research)
An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis
Using the Dot Assay to Analyze Migration of Cell Sheets
Journal (Cancer Research)
Using the Dot Assay to Analyze Migration of Cell Sheets
 
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Genomweiten RNAi-Screening, Host Faktoren zu identifizieren, die onkolytische Viren Therapie zu modulieren

Article DOI: 10.3791/56913-v • 08:51 min • April 3rd, 2018
April 3rd, 2018 •
1,2, 1,4, 1, 1, 1,2,3
1Children's Hospital of Eastern Ontario (CHEO) Research Institute, 2Department of Biology, Microbiology and Immunology, University of Ottawa, 3Department of Pediatrics, University of Ottawa, 4Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine, University of Calgary

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Hier beschreiben wir ein Protokoll für den Einsatz von Hochdurchsatz-Screening der RNAi Host Ziele aufzudecken, die manipuliert werden können, um die onkolytische Viren Therapie zu verbessern, speziell Rhabodvirus und Vaccinia-Virus-Therapie, aber es kann leicht an andere onkolytische angepasst werden Virus-Plattformen oder für die Entdeckung von Host-Gene, die Replikation des Virus in der Regel zu modulieren.

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Cancer Research Ausgabe 134 onkolytische Viren RNAi Bildschirm hohem Durchsatz Krebs "Vaccinia" Rhabdovirus Zelle Host funktionelle Genomik
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