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Journal / Immunology and Infection / Eine einfache Protokoll ortspezifisch generieren…
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Immunology and Infection
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Eine einfache Protokoll ortspezifisch generieren acetyliert Proteine in Escherichia Coli

Article DOI: 10.3791/57061-v • 11:09 min • December 9th, 2017
December 9th, 2017 •
1,2, 3, 4, 1, 1,2
1Department of Chemistry and Biochemistry, University of Arkansas, 2Cell and Molecular Biology Program, University of Arkansas, 3Department of Biological Sciences, University of Arkansas, 4Fayetteville High School

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Erweiterung des genetischen Codes dient als ein mächtiges Werkzeug für eine Vielzahl von biologischen Prozessen, einschließlich Protein Acetylierung zu studieren. Hier zeigen wir, dass eine einfache Protokoll nutzen diese Technik zur Erzeugung von homogen Proteine an bestimmten Standorten in Escherichia coli Zellen acetyliert.

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Immunologie Ausgabe 130 Protein Acetylierung Post-translationale Modifikation Erweiterung des genetischen Codes noncanonical Aminosäuren Malat-Dehydrogenase synthetische Biologie
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