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Neuroscience

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斑马鱼脑幼虫神经元、巨噬细胞和小胶质的分离和 RNA 提取
 
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斑马鱼脑幼虫神经元、巨噬细胞和小胶质的分离和 RNA 提取

Article DOI: 10.3791/57431-v 09:20 min April 27th, 2018
April 27th, 2018

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我们提出了一种在生理和病理条件下, 从斑马鱼脑中分离出神经元、巨噬细胞和小胶质的协议。分离后, 从这些细胞中提取 RNA, 分析其基因表达谱。该协议允许收集高质量的 RNA 来执行下游分析, 如 qPCR 和转录组学。

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神经科学 问题 134 转录 qPCR 小胶质细胞 神经元 RNA 提取 资产管制 斑马鱼 发展。
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