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Journal / Biochemistry / Обнаружение малых ГТФазы Prenylation и GTP…
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Biochemistry
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JoVE Journal Biochemistry
Обнаружение малых ГТФазы Prenylation и GTP привязки с помощью мембраны фракционирования и ГТФазы-Assay иммуносорбента
Extraction and Analysis of Taiwanese Green Propolis
Journal (Biochemistry)
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Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
Journal (Biochemistry)
Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
 
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Обнаружение малых ГТФазы Prenylation и GTP привязки с помощью мембраны фракционирования и ГТФазы-Assay иммуносорбента

Article DOI: 10.3791/57646-v • 13:52 min • November 11th, 2018
November 11th, 2018 •
1,2, 1,2,3, 1, 1,2, 4, 5, 6,7,8, 1,9, 1,2,10
1Department of Human Anatomy and Cell Science, Rady Faculty of Health Sciences, Max Rady College of Medicine, University of Manitoba, 2Biology of Breathing Theme, Children Hospital Research Institute of Manitoba, University of Manitoba, 3Department of Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, 4Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine, Center for Comparative Respiratory Biology and Medicine, 5Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, 6Department of Molecular Biology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, 7Centre de biophysique moléculaire - UPR 4301, Centre national de la recherche scientifique (CNRS) CS80054, 8LinkoCare Life Sciences AB, 9College of Nursing and Children's Hospital Research Institute of Manitoba, Rady Faculty of Health Sciences, University of Manitoba, 10Health Policy Research Center, Institute of Health, Shiraz University of Medical Sciences

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Здесь мы описываем протокол для исследования prenylation и 5'-гуанозинтрифосфатом (GTP)-Загрузка Rho ГТФазы. Этот протокол состоит из двух подробных методов, а именно мембраны фракционирования и связаны ГТФазы иммуноферментного анализа. Протокол может использоваться для измерения prenylation и GTP загрузки различных других малых GTPases.

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Биохимия выпуск 141 СИМВАСТАТИН глиобластома Rho ГТФазы клеточной биологии рака prenylation мевалоната путь
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