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Journal / Biochemistry / Pflanzliche Proteine in Insektenzellen…
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Biochemistry

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JoVE Journal Biochemistry
Pflanzliche Proteine in Insektenzellen abgesondert ändern Baculovirus Expressionsvektoren herstellen
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Pflanzliche Proteine in Insektenzellen abgesondert ändern Baculovirus Expressionsvektoren herstellen

Article DOI: 10.3791/58283-v • 09:14 min • August 20th, 2018
August 20th, 2018 •
1, 1, 1
1Department of Molecular and Structural Biochemistry, College of Agriculture and Life Sciences, North Carolina State University

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Hier präsentieren wir Ihnen die Protokolle zur Nutzung von Insekten Zell- und Baculovirus Protein Expressionssystems zu produzieren große Mengen an Pflanzeneiweiß abgesondert für Protein Kristallisation. Ein Baculovirus Expressionsvektor wurde entweder mit GP67 oder Insekt Hemolin Signalpeptid für Pflanze Sekretion Proteinexpression in Insektenzellen geändert.

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Biochemie Ausgabe 138 Insekt-Zelle Baculovirus Expressionsvektor sekretorischen Protein signal-Peptid GP67 Hemolin Bacmid Transfektion Zellkultur Glykosylierung Proteinexpression Protein Kristallisation
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