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Immunology and Infection

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Mundtlige Intubation af voksen zebrafisk: en Model for evaluering af tarmens optagelse af bioaktive stoffer

Article DOI: 10.3791/58366-v • 04:33 min • September 27th, 2018
September 27th, 2018 •
1,2, 1,2, 1,2
1Institute of Biotechnology and Biomedicine (IBB-UAB), Universitat Autònoma de Barcelona, 2Department of Cell Biology, Animal Physiology and Immunology, Universitat Autònoma de Barcelona

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Protokollen beskriver intubere voksen zebrafisk med en biologiske; derefter dissekere og forberede flowcytometri, Konfokal mikroskopi og qPCR tarmen. Denne metode tillader administration af bioaktive stoffer til at overvåge tarmens optagelse og den lokale immun stimulus fremkaldt. Det er relevant for afprøvning intestinal dynamikken i mundtlig forebyggelse.

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Immunologi og infektion sag 139 zebrafisk mundtlige intubation tarmen mucosal immunitet flowcytometri Konfokal imaging qPCR
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