Zebrafish Model
Zebrafish Model - Related Videos
07:58
Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury
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Cited by 8 •
2016
Renal injuries incurred from nephrotoxins, which include drugs ranging from antibiotics to chemotherapeutics, can result in complex disorders whose pathogenesis remains incompletely understood. This protocol demonstrates how zebrafish can be used for disease modeling of these conditions, which can be applied to the identification of renoprotective measures.
01:31
Zebrafish as an Alternative Vertebrate Model Organism in Biomedical Research
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2026
All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Zebrafish (Danio rerio) are widely used as an alternative vertebrate model organism in biomedical research. Their genetic similarity to humans, rapid development, and suitability for large-scale studies make them valuable tools...
02:26
Development of a Zebrafish Larval Infection Model Using Microgavage
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2026
Source: Li, J., et al. Development of a Larval Zebrafish Infection Model for Clostridioides difficile. J. Vis. Exp. (2020).This video demonstrates the microgavage procedure, where an anesthetized zebrafish larva is immobilized in agarose and orally injected with a pathogenic bacterium using a tracer-guided needle. The non-invasive delivery mimics natural infection, enabling analysis of bacterial replication, toxin release, and intestinal epithelial damage.
10:03
A Zebrafish Model of Diabetes Mellitus and Metabolic Memory
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Cited by 69 •
2013
Metabolic memory is the phenomenon by which diabetic complications persist and progress unimpeded even after euglycemia is achieved pharmaceutically. Here we describe a diabetes mellitus zebrafish model which is unique in that it allows for the examination of the mitotically transmissible epigenetic components of metabolic memory in vivo.
10:07
Assessing Teratogenic Changes in a Zebrafish Model of Fetal Alcohol Exposure
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Cited by 34 •
2012
In order to understand the molecular mechanisms of the ethanol-induced developmental damage, we have developed a zebrafish model of ethanol exposure and are exploring the physical, cellular, and genetic alterations that occur after ethanol exposure1. We then seek to find potential interventions and rapidly test them in this animal model.
FAQs
Optical transparency permits imaging of developing structures in the living embryo rather than relying only on observations after development. Researchers can follow organ formation and cellular signaling as these events occur. That visibility supports experiments linking dynamic biological processes to physiological or disease-related findings within the same research system.
Accessible genetics makes it possible to manipulate gene function in zebrafish embryos during early development. Researchers can then relate gene changes to organ formation, physiological processes, disease-related biology, or drug responses. This capability connects molecular interventions with observations in a living vertebrate, supporting mechanistic studies rather than purely descriptive comparisons.
Small size and high reproductive output make zebrafish practical for efficient screening and imaging. Researchers can examine many developing organisms while retaining a living vertebrate context, allowing developmental, physiological, or drug-response questions to be investigated at useful scale. These features support both focused experiments and broader screening studies.
A typical investigation can start with embryos developing outside the mother, allowing direct observation of organ formation. Researchers may then manipulate gene function and examine resulting developmental, physiological, or disease-related findings. This workflow combines controlled biological intervention with observation in a living organism, making it useful for connecting biological mechanisms to outcomes.
Zebrafish research can address cell signaling, regeneration, behavior, toxicology, physiology, disease, and drug responses. The appropriate use depends on whether researchers need to examine a developmental process, a whole-organism response, or effects associated with disease or drugs. This breadth makes the model relevant across multiple branches of biology.
Findings from zebrafish can guide studies in other model organisms and inform biomedical research. Their relevance comes from combining vertebrate biology with accessible observations of development, physiology, disease, and drug responses. Researchers can therefore use the system as a source of biological evidence that helps shape follow-up work beyond zebrafish.