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Aislamiento de núcleos de adultos de la médula espinal para secuenciación masivamente paralela solo núcleo ARN
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Aislamiento de núcleos de adultos de la médula espinal para secuenciación masivamente paralela solo núcleo ARN

Article DOI: 10.3791/58413-v • 06:38 min • October 12th, 2018
October 12th, 2018 •
1, 1, 2, 3,4, 3, 1
1Spinal Circuits and Plasticity Unit, National Institute of Neurological Disorders and Stroke, 2Bioinformatics Section, Information Technology Program, National Institute of Neurological Disorders and Stroke, 3Laboratory of Cochlear Development, National Institute on Deafness and Other Communication Disorders, 4Single Cell Analysis Facility, Frederick National Laboratory

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Aquí, presentamos un protocolo para aislar rápidamente a núcleos de alta calidad del tejido fresco o congelado para la descendente secuencia de RNA masivamente paralelo. Nos incluyen tejido detergente-mecánica y de mecánica hipotónica interrupción y célula opciones de lisis, que pueden utilizarse para el aislamiento de los núcleos.

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Neurociencia número 140 núcleos RNA secuenciación snRNA-Seq masivamente paralelo médula espinal gradiente de sacarosa
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