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Single-cell RNA-sekvensering av Fluorescently märkt mus nervceller med manuell sortering och dubbel In Vitro transkription med absolut räknas sekvensering (DIVA-Seq)
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Single-cell RNA-sekvensering av Fluorescently märkt mus nervceller med manuell sortering och dubbel In Vitro transkription med absolut räknas sekvensering (DIVA-Seq)

Article DOI: 10.3791/58690-v • 07:49 min • October 26th, 2018
October 26th, 2018 •
1, 1
1Cold Spring Harbor Laboratory

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Det här protokollet beskriver manuell sortering procedur för att isolera enstaka fluorescently märkt nervceller följt av in vitro- transkription-baserade mRNA förstärkning för hög-djup encelliga RNA-sekvensering.

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Neurovetenskap fråga 140 manuell sortering neuron encelliga RNA sekvensering DIVA-Seq in vitro transkription linjär förstärkning unik molekylär identifierare (UMI)
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