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Høj overførselshastighed Nitrobenzoxadiazole-mærket Cholesterol Efflux Assay
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Høj overførselshastighed Nitrobenzoxadiazole-mærket Cholesterol Efflux Assay

Article DOI: 10.3791/58891-v • 08:18 min • January 7th, 2019
January 7th, 2019 •
1, 1, 1
1Group of Genomics and Pharmacogenomics in HIV, Laboratory of Retrovirology and Viral Immunopathogenesis, IDIBAPS, Hospital Clínic de Barcelona

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Måling af in vitro-cholesterol efflux kapacitet til serum eller plasma i makrofag cell modeller er et lovende redskab som en biomarkør for åreforkalkning. I den foreliggende undersøgelse, vi optimere og standardisere en fluorescerende NBD-cholesterol efflux metode og udvikle en høj overførselshastighed analyse ved hjælp af 96-brønd plader.

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Medicin spørgsmål 143 NBD-kolesterol makrofag HDL cholesterol efflux fluorescens apolipoprotein B-forarmet serum åreforkalkning reverse kolesterol transport
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