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Pretargeted Radioimmunotherapy Based on the Inverse Electron Demand Diels-Alder Reaction

Article DOI: 10.3791/59041-v • 09:44 min • January 29th, 2019
January 29th, 2019 •
1,2, 1,2,3, 1,2,3,4
1Department of Chemistry, Hunter College of the City University of New York, 2Ph.D. Program in Chemistry, Graduate Center of the City University of New York, 3Department of Radiology, Memorial Sloan Kettering Cancer Center, 4Department of Radiology, Weill Cornell Medical College

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This protocol describes the synthesis and characterization of a trans-cyclooctene (TCO)-modified antibody and a 177Lu-labeled tetrazine (Tz) radioligand for pretargeted radioimmunotherapy (PRIT). In addition, it details the use of these two constructs for in vivo biodistribution and longitudinal therapy studies in a murine model of colorectal cancer.

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Pretargeted Radioimmunotherapy Inverse Electron Demand Diels-Alder Reaction Click Chemistry Cancer Xenographs Mouse Model Targeted Radiotherapy PRIT Surface Antigen Targeting Antibody HuA33-TCO Synthesis TCO-NHS Methylformamide Phosphate Buffered Saline Sodium Carbonate PH Adjustment
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