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Journal / Genetics / ATAC-seq Assay med låg Mitokondrie-DNA…
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Genetics

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JoVE Journal Genetics
ATAC-seq Assay med låg Mitokondrie-DNA kontaminering från primära humana CD4 + T-lymfocyter
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ATAC-seq Assay med låg Mitokondrie-DNA kontaminering från primära humana CD4 + T-lymfocyter

Article DOI: 10.3791/59120-v • 08:36 min • March 22nd, 2019
March 22nd, 2019 •
1, 1,2, 1,3
1Department of Biology, Boston University, 2Department of Computer Science and Engineering, University of California San Diego, 3Bioinformatics Program, Boston University

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Här presenterar vi ett protokoll för att utföra ett test för transposase-tillgänglig kromatin sekvensering (ATAC-seq) på aktiverade CD4 + mänskligt lymfocyter. Protokollet har modifierats för att minimera kontaminering Mitokondrie DNA läsningar från 50% till 3% genom införandet av en ny lyseringsbuffert.

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Genetik fråga 145 Epigenomik ATAC-seq Tn5 CD4 + lymfocyter kärnor mitokondriella DNA förorening
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