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Immunology and Infection

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In Vitro ELISA-Test zur Bewertung der Wirksamkeit von Tollwut-Impfstoffen
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In Vitro ELISA-Test zur Bewertung der Wirksamkeit von Tollwut-Impfstoffen

Article DOI: 10.3791/59641-v • 09:04 min • May 11th, 2020
May 11th, 2020 •
1, 1,2
1Unit Antiviral Strategies, OIE Reference Laboratories for Rift Valley Fever Virus & Crimean Congo Hemorrhagic Fever Virus, Institut Pasteur, 2Institut Pasteur de Guinée, Conakry

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Hier beschreiben wir eine indirekte ELISA-Sandwich-Immunocapture zur Bestimmung des immunogenen Glykoproteingehalts in Tollwut-Impfstoffen. Dieser Test verwendet einen neutralisierenden monoklonalen Antikörper (mAb-D1), der Glykoproteintrimer erkennt. Es ist eine Alternative zum in vivo NIH-Test, um die Konsistenz der Impfstoff-Potenz während der Produktion zu verfolgen.

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Immunologie und Infektion Ausgabe 159 Tollwut-Impfstoff-Potenz NIH-Test ELISA-Methode mAb-D1-Monoklonaler Antikörper Trimers von Glykoprotein Glykoproteingehalt
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